Dados do Trabalho

Título

BIOACTIVES FROM SOIL SCREENED BY FUNCTION- AND SEQUENCE-DRIVEN APPROACHES AIMING BIOREMEDIATION OF PETROLEUM CONTAMINATED AREAS

Resumo

Spills of heavy oil and its derivatives in the oceans and soils are the result of different practices involving the oil production and application chain. Two major groups of hydrocarbons make up petroleum and its derivatives: aliphatic and aromatic. Among aromatic hydrocarbons, Polycyclic Aromatic Hydrocarbons (PAHs) stand out as the most toxic compounds both in their water-soluble and insoluble forms. Several studies show the bioremediation of petroleum residues as an effective biological method in different environments. Aiming the prospecting of biological resources from soil to be applied on bioremediation, we combined function- and sequence-driven approaches. Microorganisms isolated or in natural consortia were obtained from soils contaminated with lubricating oil residue, incubated in Winogradsky Columns (WC) for 44 days. Biochemical tests were performed in which the consortia were challenged in the presence of diesel oil and kerosene. For the sequence-driven approach, dataset from the Kyoto Encyclopedia of Genes and Genomes (KEGG) databank was prospected to establish an artificial consortium of bacteria. Four from 15 alive microbial bacterial consortia were selected from WC showing prominent abilities in oil degradation for further analyses. Morphological characteristics of bacterial isolates were diverse and based on color, shape, elevation and edge following Biotech Publications (Geneva, NY) and gram staining. From 50 bacterial isolates, 20 different types were identified predominantly gram-negative (40 isolates). Database analysis showed that Polaromonas naphthalenivorans seems to be the bacterium that has enzymatic resources for the degradation of a greater diversity of PAHs. This type of analysis, involving approaches guided by function and DNA sequence, allows expanding future research in the field of synthetic biology with an emphasis on genetic and metabolic engineering in which gene circuits can be assembled to configure new metabolic routes

Palavras-chave

Bacterial consortia, Polycyclic Aromatic Hydrocarbons, Biodegradation.

Instituição financiadora

Nonino Company.